An experiment to look at the effect of 1 factor on an enzyme

Aim To design and carry out an experiment to grimace at one factor which affects the enzyme catalase. Catalase is involved in the disruption of total heat Peroxide into water and atomic crook 8. 2H202 _ 2H20 + 02 It can be found in most cells, and it is mostly concentrated in the liver, kidney and genus Tuber cells of plants (e.g. stump spud). Hypothesis I predict that as the temperature increases the atomic number 8 produced obturate increase until a point (optimum temperature), then provide settle until no oxygen is produced ( due to denaturation ) Justification All enzymes defy optimum conditions, at which they work best. therefrom in my experiment there ordain be an optimum temperature i.e. the temperature where oxygen is apt(p) off most rapidly. I stated that at a temperature no oxygen would be produced. This is because I cogitate the enzyme bequeath denature due to the high temperature ( ie the tertiary organise exit change ). Due to t he denaturation of the enzyme, no oxygen will be produced. I also feel it is important to desire note that the H2O2 is an unstable compound and at high temperatures (100aC) it will disintegrate by itself.

Apparatus For my experiment I will need the chase frame-up: h Conical flaskful with side on delivery outlet h hateful fitting bung for the conic flask h rescue tube h Gas-syringe h Water bathroom h Retort stand h Boss and clamp I will set the apparatus up in the pastime way ¡V Control of variables FactorControl TemperatureI have elect to convert this factor using an electronic water bath in my experi ment. ConcentrationI will use the substrate ! from the same bottleful without dilution. I will take my cuttings of potato from the same region. Surface AreaI will use the same cork borer and cut every(prenominal) of the potato to the... If you want to get a full essay, ordination it on our website: BestEssayCheap.com

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